Journal: Neurobiology of disease

Article Title: Excessive expression of progranulin leads to neurotoxicity rather than neuroprotection.

doi: 10.1016/j.nbd.2025.106895

Figure Lengend Snippet: Fig. 2. Decline in motor function of PGRN Tg mice. (A, B) Hindlimb clasping test. (A) When the tail lifted mice, the non-Tg male mouse spread their hindlimbs outward from the abdomen (left panel), while the WT- PGRN Tg male mouse clasped their hindlimbs (right panel). (B) Clasping rates of non-Tg and WT-PGRN Tg mice. The initial number was 65 for non-Tg males, 45 for WT-PGRN Tg males, 53 for non-Tg females, and 43 for WT-PGRN Tg females. (C) Rotarod test (8-month-old). The latency to fall from the rotating drum was measured 3 times a day. Data indicated by plots are means of 3 trials per day ± SEM. The number of mice was 15 for non-Tg males and 11 for WT-PGRN Tg males. **p < 0.01 (two-way ANOVA, Genotype: F (1, 72) = 18.86). (D) Grip strengths of non-Tg or WT-PGRN Tg (8-month-old). Scatter plot graphs show individual data. Data indicated by bars are means ± SEM; the number of mice was 15 for non-Tg males and 11 for WT-PGRN Tg males. *p < 0.05 (Student's t-test). (E) Frozen cerebellum sections from non-Tg or WT-PGRN Tg male mice (6-month-old) were immunostained with anti-Calbindin, Parvalbumin, GFAP, Iba1, or Lamp1 antibodies. The photograph within the white line is an enlarged image of the area enclosed by the dotted line. Scale bar, 100 μm. (F) Autofluorescence signals in the cerebellum of non-Tg or WT-PGRN Tg male mice (6-month-old). The photograph within the white line is an enlarged image of the area enclosed by the dotted line. Scale bar, 100 μm.

Article Snippet: A rabbit anti-Glial fibrillary acidic protein (GFAP) pAb (Cat# 16825-1-AP, RRID: AB_2109646) was from Proteintech (Rosemont, IL, USA).

Techniques:

Journal: Neurobiology of disease

Article Title: Excessive expression of progranulin leads to neurotoxicity rather than neuroprotection.

doi: 10.1016/j.nbd.2025.106895

Figure Lengend Snippet: Fig. 3. PGRN Tg mice show a cognitive decline but no hippocampal neuronal loss. (A) Coronal sections of the hippocampus (CA3 or CA1 region) or cerebral cortex from non-Tg or WT-PGRN Tg male mice (12-month-old) were immunostained with anti-NeuN antibody. Scale bar, 100 μm. (B) Coronal hippocampus or cerebral cortex sections from non-Tg or WT-PGRN Tg male mice (12-month-old) were immunostained with anti-GFAP, Iba1, or Lamp1 antibodies. The photograph within the white line is an enlarged image of the area enclosed by the dotted line. Scale bar, 100 μm. (C, D) Open-field test. The number of rearing, the total distance traveled, and the time spent in the center (left to right panels, respectively) were recorded automatically. Scatter plot graphs show individual data. Data indicated by bars are means ± SEM of each group. The number of 8-month-old mice (C) was 15 for non-Tg males, 11 for WT-PGRN Tg males, and the number of 12-month-old mice (D) was 21 for non-Tg males and 19 for WT-PGRN Tg males. *p < 0.05, ***p < 0.001 (Student's t-test). (E) Novel object recognition test (12-month-old) of male. The time spent around familiar (Fam) or novel (Nov) object was recorded automatically. Scatter plot graphs show individual data. Data indicated by bars are means ± SEM of each group. The number of mice was 13 for non-Tg males and 10 for WT-PGRN Tg males. *p < 0.05 (Student's t-test).

Article Snippet: A rabbit anti-Glial fibrillary acidic protein (GFAP) pAb (Cat# 16825-1-AP, RRID: AB_2109646) was from Proteintech (Rosemont, IL, USA).

Techniques:

Journal: Neurobiology of disease

Article Title: Excessive expression of progranulin leads to neurotoxicity rather than neuroprotection.

doi: 10.1016/j.nbd.2025.106895

Figure Lengend Snippet: Fig. 4. Overexpression of PGRN does not influence differentiation into neurons. (A) Frozen cerebellum sections from non-Tg or WT-PGRN Tg male mice (1-month-old) were immunostained with anti-PGRN, Calbindin, Parvalbumin, GFAP, or Iba1 antibodies. The photograph within the white line is an enlarged image of the area enclosed by the dotted line. Scale bar, 100 μm. (B, C) Frozen sections of the cerebral cortex (Ctx), hippocampus (Hip), and cerebellum (CB) from non-Tg or WT-PGRN Tg male mice (3-, 6- or 12-month-old), as well as 1-month-old mice, were immunostained with anti-GFAP or Iba1 antibodies, and GFAP-positive (GFAP+) area and Iba1-positive (Iba1+) puncta were measured. (B) The fold increase of GFAP+

Article Snippet: A rabbit anti-Glial fibrillary acidic protein (GFAP) pAb (Cat# 16825-1-AP, RRID: AB_2109646) was from Proteintech (Rosemont, IL, USA).

Techniques: Over Expression

Journal: Journal of Nanobiotechnology

Article Title: Tungsten-based polyoxometalate nanoclusters as ferroptosis inhibitors modulating S100A8/A9-mediated iron metabolism pathway for managing intracerebral haemorrhage

doi: 10.1186/s12951-025-03149-9

Figure Lengend Snippet: W-POM NCs inhibit ICH-induced neuroinflammation, oxidative stress and ferroptosis in ICH mice model. ( a ) Protein expression of iron transport-related proteins (TFR1, FPN, DMT1) assessed using western blot. ( b - d ) Statistical graph of grayscale values of TFR1, FPN, DMT1 protein expression. ( e ) Representative TEM images of mitochondrial morphology of the neurons in the perihaematomal area at 72 h after ICH. Red arrows indicate mitochondria. Representative images by Nissl staining ( f ) and FJB staining ( g ) in the perihaematomal tissue showing the effects of W-POM NCs on ICH-induced neuronal injury and neurodegeneration. Statistical analysis of Nissl staining ( h ) and FJB staining ( i ) results in the perihaematomal tissue. ( j ) Representative images of immunofluorescent staining for Iba-1 (red) and DAPI (blue) in the perihaematomal area at 72 h after ICH. ( l ) Quantitative analyses of Iba-1 positive cells in the perihaematomal area at 72 h after ICH. ( k ) Representative images of immunofluorescent staining for GFAP (red) and DAPI (blue) in the perihaematomal area at 72 h after ICH. ( m ) Quantitative analyses of GFAP-positive cells in the perihaematomal area at 72 h after ICH. (* P < 0.05, ** P < 0.01, *** P < 0.001, means ± SEM). ICH, intracerebral haemorrhage; TEM, transmission electron microscopy; W-POM NCs, tungsten-based polyoxometalate nanoclusters; TFR1, transferrin receptor 1; DMT1, divalent metal transporter 1; FPN, ferroportin

Article Snippet: The following primary antibodies were used: rabbit anti-Iba1 polyclonal antibody (1:500, GB113502, Servicebio, China) and rabbit anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:200, CL488-16825, Proteintech, China).

Techniques: Expressing, Western Blot, Staining, Transmission Assay, Electron Microscopy